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Denaturation

Fruit? Egg? Fish? What do they all have in common? Besides being food, they are also packed with proteins. Proteins perform many vital functions in our bodies. Proteins can keep structure in our bodies and foods. For instance, when we whisk eggs into a cake recipe, we're doing so to 1) bind the ingredients together and 2) give the cake its texture. We've also come across proteins, including yogurt and milk casein proteins. Proteins are in our bodies and foods, performing essential functions, making it necessary to learn about them.  

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Denaturation

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Fruit? Egg? Fish? What do they all have in common? Besides being food, they are also packed with proteins. Proteins perform many vital functions in our bodies. Proteins can keep structure in our bodies and foods. For instance, when we whisk eggs into a cake recipe, we're doing so to 1) bind the ingredients together and 2) give the cake its texture. We've also come across proteins, including yogurt and milk casein proteins. Proteins are in our bodies and foods, performing essential functions, making it necessary to learn about them.

So, without further ado, let's dive into proteins and protein denaturation!

  • First, we will look at the definition of protein denaturation.
  • Then, we will talk about the causes of denaturation.
  • After, we will explore the characteristics of denaturation.
  • Then, we will talk about the significance of protein denaturation.
  • Lastly, we will look at some examples related to protein denaturation.

Protein Denaturation

Let's start by looking at the definition of proteins.

Proteins are organic compounds consisting of small molecules called amino acids.

  • Organic compounds are compounds that contain mainly carbon and can sustain life. Organic compounds are also commonly made of hydrogen, oxygen, or nitrogen.

Amino acids are proteins' building blocks or monomers, as shown in Figure 1. They consist of an alpha (\(\alpha\)) carbon bonded to an amino group (\(NH_2\)), a carboxyl group (\(COOH\)), hydrogen (\(H\)), and a variable side chain named (\(R\)) which gives it different chemical properties.

Proteins are essential to life as they perform a variety of functions. They can transport materials, control physiological processes such as growth, speed up chemical reactions, store things, etc.

Denaturation Amino Acid Structure Diagram StudySmarterFigure 1: Amino acid structure. Daniela Lin, Study Smarter Originals.

Featured on the table below are some common examples of proteins:

Types of Proteins
Functions
Example
Enzymes Enzymes catalyze and speed up reactions. Amylase breaks down sugars and starches.
StructuralStructural proteins maintain cell shape and structure. Keratin strengthens hair and nails.
Transport Move materials around the body. Hemoglobin carries oxygen around the body.
Defense They protect your body by maintaining barriers or eliminating threats. Antibodies bind to foreign molecules (antigens) to facilitate their removal.

Proteins come in different sizes and shapes, and the shape of proteins is essential for their functions. There are generally two shapes of proteins: globular and fibrous.

  • Globular proteins are spherical, usually act as enzymes or transport materials, are generally soluble in water, have an irregular amino acid sequence, and are usually more sensitive to heat and pH changes than fibrous ones. A globular protein is a hemoglobin, as shown in Figure 2.

  • Fibrous proteins are narrower and more prolonged, usually are structural in function, are generally not soluble in water, have a regular amino acid sequence, and are usually less sensitive to heat and pH changes than globular ones. An example of a fibrous protein is keratin, as shown in Figure 2.

Denaturation Illustration of Protein Shapes StudySmarterFigure 2: Examples of different protein shapes. Daniela Lin, Study Smarter Originals.

Globular proteins are more soluble than fibrous because the amino acids can bend in a way where the polar groups are on the surface. Globular proteins also have weaker interactions between their amino acid sequences when compared to fibrous proteins making them easier to denature.

When a protein loses its shape, we say it denatures.

Denaturation involves breaking bonds in protein structure, typically due to heat.

Denaturation often results in loss of protein shape, structure, function, and eventual degradation.

Causes of Denaturation

Now that we know what protein denaturation is, we can ask what causes a protein to denature.

  • Temperature changes or heat increases result in molecule vibrations that break bonds in proteins leading to denaturation. Note that denaturation because of cold temperature also occurs; it's harder to detect as it happens below freezing temperatures or below \(0^\circ C\).

  • pH changes lead to an acid-base imbalance or different amounts of hydrogen and hydroxide ions which can affect the bonding in proteins resulting in denaturation.

Other physical and chemical changes in a protein's environment can also lead to denaturation:

  • Addition of chemical agents, such as urea. Urea leads to protein denaturation because it forms stronger hydrogen bonds with the protein backbone than water does.

  • Addition of metal poisons like lead. Metals like lead interact with a protein's calcium-binding sites leading to the breakage of bonds or denaturation.

Physical agents are non-chemical ways that can result in injury or risk to human health.

Chemical agents are chemical compounds with toxic health effects on humans.

All common factors that can cause denaturation are shown in the table below:

Chemical Agents
Physical Agents
Detergents (ex) charged detergents such as sodium dodecyl sulfate (SDS)UV light
Organic solvents (ex) alcoholHigh pressures
pH Extreme agitations
Inorganic compounds (ex) urea Heat

Characteristics of Denaturation

After understanding what protein denaturation is and why proteins denature, we must go over what parts of a protein can denature. We find this by understanding how a protein's final conformation is created.

As we discussed earlier, proteins are made of a chain of amino acids. When a few chains of amino acids bind together, they create peptide bonds. Longer chains of amino acids bound together are called polypeptide bonds. All structures mentioned below are shown in Figure 3 except the (\(\beta\)) pleated sheets.

  • Primary structure: A protein's primary structure is its amino acid sequences linked into a polypeptide chain. This sequence determines a protein's shape.

  • Secondary structure: The secondary structure is caused by folding amino acids from the primary structure. The most common structures proteins fold into in the secondary level are alpha (\(\alpha\)) helices and beta (\(\beta\)) pleated sheets, which are held together by hydrogen bonds.

  • Tertiary structure: The tertiary structure is a protein's three-dimensional structure. This three-dimensional structure is formed by the interactions between the variable R groups.

  • Quaternary structure: Not all proteins have a quaternary structure. But some proteins can form quaternary structures that consist of multiple polypeptide chains. These polypeptide chains can be referred to as subunits

Denaturation Illustration of Protein Structures StudySmarterFigure 3: Protein structure (primary, secondary, tertiary, and quaternary). Daniela Lin, Study Smarter Originals.

Each protein has its own sequence and shapes with chemical interactions that bond it together. Denaturation occurs when there are physical and chemical changes to the environment, including but not limited to temperature, pH, and the addition of chemicals. Denaturation often involves the breakdown of every structure except the primary structure.

In general, denatured proteins have a less uniform, loose structure which is usually insoluble in water.

The denaturation of proteins destroys a protein's three-dimensional or tertiary structure and secondary structure (also quaternary if a protein has more than one polypeptide chain).

Whether a protein can fold back after denaturation depends on the complexity and type of the protein itself. The reason "renaturation" can occur is that the primary structure of a protein isn't destroyed. Some proteins have all the instructions needed for folding in their amino acid sequence. However, other proteins might need chaperones.

Renaturation is the process by which proteins reform to their original confirmation, usually after denaturation.

Chaperones are proteins that aid in the folding or unfolding of bigger proteins or proteins with complexes.

However, in extreme conditions, renaturation isn't possible. For instance, when we boil or fry an egg, we put it through so much heat that renaturation of the protein can't occur.

Have you ever wondered if there are practical ways to renature proteins? Well, the answer is yes. Scientists can use microspheres to attempt to renature proteins.

Microspheres are small, hollow particles that are circular and usually made from glass or ceramic.

For instance, misfolded ribonuclease A, which catalyzes RNA degradation to confer greater host immunity, can be renatured or refolded using microspheres. After placing them in microspheres, we can introduce disulfide and sulfhydryl groups. Scientists theorize that the sulfhydryl groups interact with the disulfide ones and lead to protein refolding as thiol-disulfide reactions occur, whereby thiol is oxidized to form disulfide bonds.

Disulfide bonds (\(S-S\)) are the interactions that stabilize the three-dimensional protein formation or the tertiary structure, and they are usually formed between two cysteines.

Cysteine is one of the 20 amino acids living organisms use.

Significance of Protein Denaturation

When proteins denature, they stop functioning, and this is because the protein's shape and conformation determine a protein's function.

When proteins stop functioning, they can't transport materials, signal molecules, support structures in our bodies, and more! Proteins also help repair and make new cells essential for survival.

But besides being the building blocks of our lives, proteins can also be used in novel drug designs. Designing drugs involves designing molecules whose shapes complement our biological target. This is because drugs usually work by binding to the target's receptor site or where they either block the protein's effect or copy it. Sometimes drugs can bind to multiple targets' receptor sites.

Scientists think that by binding to multiple targets' receptor sites, drugs can create a "molecular interaction signature" that can be used to identify new drugs or new uses for old/existing drugs.

Denaturation Examples

Now, let's explore some examples of denaturation.

Eggs

Eggs are made of both water and protein. We're denaturing the protein halfway when we whip egg whites to get air into them, creating a light and fluffy consistency perfect for desserts. If the egg whites get beaten, they will eventually denature all the way and become stiff.

Molecularly, when the egg whites are being beaten, the protein unravels so that the hydrophilic ends bond with water, and the hydrophobic parts go towards the air. This creates bonds that hold the whipped mixture and air bubbles in place. If they aren't beaten until stiff, then the proteins are only half-denatured, which means they will retain their elastic properties. When baked, the proteins will denature completely and surround the air bubbles trapping them, thereby creating a light and fluffy consistency in some desserts like souffle.

Hydrophilic substances have a tendency or like to be in the water. In other words, they have a strong affinity to water or are "water-loving"—for example, oils.

Hydrophobic substances tend not to like water. In other words, they are "water-hating" or won't mix with water—for example, oil and fats.

  • Polar amino acids are hydrophilic because water is also a polar molecule. Polar means a molecule has charged parts that are not canceled out (either - or +).
  • Nonpolar amino acids are hydrophobic. Nonpolar means amino acids that usually have canceled-out charges or where the variable R group is composed of hydrogens and carbons.

Meat

Cooking meat involves denaturation by heat, making it not reversible at all. This process accompanies color changes. For example, when we cook a steak, structural changes happen. The connective tissues and muscle proteins start to denature, changing meat color and tenderness.

But precisely what are some proteins involved in this process?

  • Myoglobin is what gives meat its reddish hue. Due to myoglobin denaturation, the color changes around 60 \(^\circ C\).

  • Meat changes from see-through to opaque as the proteins denature because the protein unravels and coagulates, making light unable to pass.

  • Myosin, a fibrous protein, is responsible for the changes in meat texture from raw to tender around 40 \(^\circ C\).

  • Actin, another fibrous protein, denatures at higher temperatures than myosin. It denatures around ~70 \(^\circ C\), and it's responsible for making meat tougher and drier.

Denaturation - Key takeaways

  • Proteins are organic compounds consisting of small molecules called amino acids.
  • Proteins are essential to life as they perform a variety of functions. They can transport materials, control physiological processes such as growth, speed up chemical reactions, store things, etc.
  • Denaturation often results in loss of protein shape, structure, function, and eventual degradation.

  • Each protein has its own sequence and shapes with chemical interactions that bond it together. Denaturation occurs when there are physical and chemical changes to the environment.

  • Whether a protein can fold back after denaturation depends on the complexity and type of the protein itself. The reason "renaturation" can occur is that the primary structure of a protein isn't destroyed.


References

  1. http://pressbooks-dev.oer.hawaii.edu/humannutrition/chapter/the-role-of-proteins-in-foods-cooking-and-denaturation/
  2. https://www.youtube.com/watch?v=YhTpnRzp-eQ
  3. https://www.youtube.com/watch?v=hok2hyED9go
  4. https://chem.libretexts.org/Courses/University_of_Arkansas_Little_Rock/Chem_4320/Chem_4320%2F%2F5320%3A_Biochemistry_1/02%3A__Protein_Structure/2.3%3A_Denaturation_of_proteins
  5. https://openstax.org/books/biology-2e/pages/3-4-proteins
  6. https://www.sciencedirect.com/science/article/abs/pii/S0927775798006256?via%3Dihub
  7. https://www.youtube.com/watch?v=NGPcJgFLfUc
  8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155886/
  9. https://www.exploratorium.edu/cooking/icooks/11-03-03.html#:~:text=If%20egg%20whites%20are%20beaten,retain%20some%20of%20their%20elasticity.
  10. https://blog.thermoworks.com/beef/coming-heat-effects-muscle-fibers-meat/

Frequently Asked Questions about Denaturation

Denaturation involves breaking bonds in protein structure, usually due to heat. 

Denaturation of an enzyme would result in loss of protein shape, structure, function, and eventual degradation. This means that enzymes would no longer catalyze reactions. 

Denaturation often results in loss of protein shape, structure, function, and eventual degradation.  

Multiple physical and chemical changes in a protein's environment can cause denaturation, including but not limited to temperature and pH changes, metal poisons, organic solvents, etc.

The denaturation of proteins destroys a protein's three-dimensional or tertiary structure (and also quaternary if a protein has more than one polypeptide chain). It also eliminates the secondary structure, leaving only the primary form intact, leading to possible renaturation depending on the degree of denaturation.

Final Denaturation Quiz

Denaturation Quiz - Teste dein Wissen

Question

What are proteins?

Show answer

Answer

Proteins are organic compounds consisting of small molecules called amino acids.  

Show question

Question

What are organic compounds?

Show answer

Answer

Organic compounds are compounds that contain mainly carbon and can sustain life. Organic compounds are also commonly made of hydrogen, oxygen, or nitrogen. 

Show question

Question

What are amino acids?

Show answer

Answer

Amino acids are proteins' building blocks or monomers. They consist of an alpha carbon bonded to an amino group, a carboxyl group, hydrogen, and a variable side chain named, which gives it different chemical properties. 

Show question

Question

Why are proteins essential to life? 

Show answer

Answer

Proteins are essential to life as they perform a variety of functions. They can transport materials, control physiological processes such as growth, speed up chemical reactions, store things, etc. 

Show question

Question

Why are proteins important in drug design?

Show answer

Answer

Designing drugs involves designing molecules whose shapes complement our biological target. This is because drugs usually work by binding to the target's receptor site or where they either block the protein's effect or copy it. Sometimes drugs can bind to multiple targets' receptor sites. 


Scientists think that by binding to multiple targets' receptor sites, drugs can create a "molecular interaction signature" that can be used to identify new drugs or new uses for old/existing drugs. 

Show question

Question

What is a practical way to renature proteins?

Show answer

Answer

Modified microspheres can be used to attempt to renature proteins. 

Show question

Question

What structures do all proteins have?

Show answer

Answer

primary structure

Show question

Question

What are some agents we can use to denature proteins? 

Show answer

Answer

changing temperatures 

Show question

Question

What does denaturation affect?

Show answer

Answer

Denaturation affects a protein's primary, secondary, and tertiary structure. It also influences a protein's quaternary structure if it possesses one. 

Show question

Question

How do denatured proteins compare to normal ones?

Show answer

Answer

Denatured proteins have a less uniform, loose structure which is usually insoluble in water. 

Show question

Question

Why can renaturation sometimes occur?

Show answer

Answer

The reason "renaturation" can occur is that the primary structure of a protein isn't destroyed. Some proteins have all the instructions needed for folding in their amino acid sequence.

Show question

Question

What is the reason that globular and fibrous proteins are different?

Show answer

Answer

Globular proteins are more soluble than fibrous because the amino acids can bend in a way where the polar groups are on the surface. Globular proteins also have weaker interactions between their amino acid sequences when compared to fibrous proteins making them easier to denature. 

Show question

Question

What are some functions of proteins? 

Show answer

Answer

Enzymes catalyze and speed up reactions. 

Show question

Question

What can cause protein denaturation?

Show answer

Answer

metal poisoning 

Show question

Question

What are hydrophilic and hydrophobic substances? 

Show answer

Answer

Hydrophilic substances have a tendency or like to be in the water. In other words, they have a strong affinity to water or are "water-loving"—for example, oils. 

Show question

Question

What are the proteins involved in meat denaturing? 

Show answer

Answer

myoglobin

Show question

Question

What are some critical bonds in proteins? 

Show answer

Answer

disulfide bonds

Show question

Question

How did scientists renature ribonucleases?

Show answer

Answer

Scientists renatured ribonuclease A by placing them in microspheres. They introduced disulfide and sulfhydryl groups. Scientists theorized that the sulfhydryl groups interact with the disulfide ones and lead to protein refolding as thiol-disulfide reactions occur whereby thiol is oxidized to form disulfide bonds. 

Show question

Test your knowledge with multiple choice flashcards

What are proteins?

What are organic compounds?

What are amino acids?

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Flashcards in Denaturation18

Start learning

What are proteins?

Proteins are organic compounds consisting of small molecules called amino acids.  

What are organic compounds?

Organic compounds are compounds that contain mainly carbon and can sustain life. Organic compounds are also commonly made of hydrogen, oxygen, or nitrogen. 

What are amino acids?

Amino acids are proteins' building blocks or monomers. They consist of an alpha carbon bonded to an amino group, a carboxyl group, hydrogen, and a variable side chain named, which gives it different chemical properties. 

Why are proteins essential to life? 

Proteins are essential to life as they perform a variety of functions. They can transport materials, control physiological processes such as growth, speed up chemical reactions, store things, etc. 

Why are proteins important in drug design?

Designing drugs involves designing molecules whose shapes complement our biological target. This is because drugs usually work by binding to the target's receptor site or where they either block the protein's effect or copy it. Sometimes drugs can bind to multiple targets' receptor sites. 


Scientists think that by binding to multiple targets' receptor sites, drugs can create a "molecular interaction signature" that can be used to identify new drugs or new uses for old/existing drugs. 

What is a practical way to renature proteins?

Modified microspheres can be used to attempt to renature proteins. 

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