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Mycoplasma

Navigate the intricacies of Mycoplasma with this comprehensive guide that illuminates the subject from a microbiological perspective. Explore the major types of Mycoplasmas, including Genitalium and Pneumoniae, and gain unique insights into recognising and treating Mycoplasma infections. Learn about the testing procedures and the importance of early detection, while focusing on prevention and management techniques. Stay informed with recent research developments for enhanced understanding and keep abreast of knowledge in this significant area of microbiology.

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Mycoplasma

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Navigate the intricacies of Mycoplasma with this comprehensive guide that illuminates the subject from a microbiological perspective. Explore the major types of Mycoplasmas, including Genitalium and Pneumoniae, and gain unique insights into recognising and treating Mycoplasma infections. Learn about the testing procedures and the importance of early detection, while focusing on prevention and management techniques. Stay informed with recent research developments for enhanced understanding and keep abreast of knowledge in this significant area of microbiology.

Understanding Mycoplasma: An Overview

Mycoplasma is a genus of bacteria that lack a cell wall around their cell membrane. This unique characteristic makes them resistant to many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic and are found in nearly every habitat where there is life.

The Role of Mycoplasma in Microbiology

Mycoplasma plays a critical role in microbiology, revealing key insights about bacterial life and antibiotic resistance.

Interestingly, the lack of a cell wall in these bacteria lends them certain advantages such as resistance to certain antibiotics and a greater ability to survive in various habitats.

Mycoplasma bacteria, despite their simple structure, are capable of complex interactions with host organisms. Consider this example:

Many species of Mycoplasma are found in the respiratory and urogenital tracts of humans. Under certain conditions, they can cause disease such as pneumonia and pelvic inflammatory disease. Yet, in other cases, they can exist as commensal organisms, causing no harm to the host.

In the context of molecular biology research:
Mycoplasma contamination is a serious concern for biologists.
It can alter cell properties, affect cellular responses, and invalidate experiment results.
Thus, regular testing for contamination is crucial in a laboratory setting.

Major Types of Mycoplasma: Genitalium and Pneumoniae

There are nearly 200 known species of Mycoplasma, but the two most well-studied are Mycoplasma genitalium and Mycoplasma pneumoniae.
  • Mycoplasma genitalium was first isolated from the human urogenital tract.
  • Mycoplasma pneumoniae is a significant cause of atypical pneumonia.
Details on Mycoplasma genitalium and Mycoplasma pneumoniae are tabulated as follows:
SpeciesResearch Significance
Mycoplasma genitaliumIt has one of the smallest genomes of any free-living organism, making it a model organism for minimal cell studies.
Mycoplasma pneumoniaeStudy of this bacterium has brought important insights into bacterial pathogenesis, especially host-adaptation mechanisms.
A feature that you would find fascinating is the different replication timings for these Mycoplasma species. For instance, Mycoplasma genitalium has a lengthy replication time as documented in this equation \( T_{replication} = 12 \, hours \), while Mycoplasma pneumoniae has a considerably faster replication period \( T_{replication} = 1 \, hour \). Understanding Mycoplasma's mechanisms of interaction with their host and adaptation capabilities has the potential to provide exciting insights into microbial life and evolution.

Mycoplasma Infections: A Closer Look

In this section, you'll delve further into the fascinating world of Mycoplasma, this time focusing on infections caused by these tiny bacteria.

Recognising Mycoplasma Symptoms: Key Indicators

Mycoplasma infections can manifest with various symptoms, depending on the species involved and the area of the body affected. Mycoplasma pneumoniae, for example, typically causes respiratory infections. Here, you may see symptoms similar to those of a common cold or flu initially, with persistent dry cough, sore throat, headache, and gradual onset fever. As the infection progresses, additional manifestations like chest pain and laboured breathing may develop. However, in the case of Mycoplasma genitalium, the infection primarily impacts the urogenital tract. In men, it can lead to symptoms such as urethritis (inflammation of the urethra) with symptoms including burning sensation during urination and penile discharge. For women, it can cause cervicitis (inflammation of the cervix) with symptoms like abnormal vaginal discharge and post-coital bleeding. Besides these species-specific manifestations, general unexplained fatigue and body aches could also indicate a Mycoplasma infection, especially in atypical cases where conventional diagnoses do not yield a cause.

Mycoplasma Infection: Causes and Treatment

A Mycoplasma infection occurs when you are exposed to the bacteria, often through respiratory droplets (from a cough or sneeze) or sexual contact, depending on the species of Mycoplasma.

The uniqueness of Mycoplasma also reflects in their treatment methodologies. Since they are devoid of a cell wall, typical antibiotics such as penicillin, that target cell wall synthesis, are ineffective against them.

Instead, antibiotics that target the protein synthesis mechanism of the bacteria, like macrolides (Azithromycin, Clarithromycin) or tetracyclines (Doxycycline), are used. Additionally, quinolones like Moxifloxacin may also be effective. Treatment duration can vary from 5-14 days, depending on the clinical response.

The Impact of Mycoplasma Hominis Infections

Mycoplasma hominis is another key species that primarily inhabits the urinary and genital tracts of humans. While it is often harmless, it can become problematic under certain conditions. In women, Mycoplasma hominis can cause pelvic inflammatory disease if it ascends into the reproductive organs. It can also lead to infection of the amniotic fluid (amnionitis) and postpartum fever in pregnant women, potentially endangering both the mother and the foetus. In men, this bacterium can lead to non-gonococcal urethritis, similar to Mycoplasma genitalium. What makes Mycoplasma hominis truly challenging, though, is the bacterium's resistance to many antibiotics, including tetracyclines. This antibiotic resistance results from the transfer of resistance genes from one bacterium to another, a phenomenon noted by the following equation: \( Antibiotic \, Resistance = Genes_{transfer} \times Bacteria_{number} \). Treatment usually includes antibiotics such as macrolides or fluoroquinolones, although the choice may be complicated by the fact that Mycoplasma hominis is naturally resistant to several antibiotics. Understanding the mechanisms of Mycoplasma infection and targeting specific paths of pathogenesis and resistance are critical in managing these infections.

Delving Into Mycoplasma Testing

Diagnostic testing for Mycoplasma infection is an area of immense importance in the medical field. Given the subtle presentation of many Mycoplasma infections, accurate and timely diagnosis is crucial.

How are Mycoplasma Tests Conducted?

The test for Mycoplasma typically involves the collection of a clinical specimen. Depending on the suspected site of infection, this could involve a sputum sample, a throat swab, a urine sample, or a swab from the affected genital area. The collected samples are then subjected to several laboratory tests. The first is usually a culture test to identify the growth of Mycoplasma bacteria. Since Mycoplasma requires specific conditions to grow, it is cultured on a selective medium, a process which may take up to a week. Next, there are several other test methods that can be applied:
  • Serological Tests: These detect the body's immune response to Mycoplasma infection. By measuring the level of antibodies (either IgM or IgG), these tests identify the presence of an active or recent infection. However, these are not species-specific and can create interpretative complications.
  • Molecular Tests: These involve the identification of Mycoplasma DNA or RNA in the patient's sample. Polymerase chain reaction (PCR) is a common molecular test that provides rapid and highly sensitive detection of Mycoplasma species, even in early stages of infection.

Understanding the Results of a Mycoplasma Test

Interpreting the results of a Mycoplasma test requires a clear understanding of the nature of these bacteria and the way they interact with the human host. A positive culture test for Mycoplasma indicates active infection. This is typically identified by characteristic 'fried-egg appearance' colonies under microscopic examination. However, because Mycoplasma can sometimes exist harmlessly in individuals, especially in the urogenital tract, the clinical presentation must also be considered. Positive results for the PCR test indicate the presence of Mycoplasma DNA or RNA in the sample, providing strong evidence for an active infection. In serologic tests, the presence of IgM antibodies suggest an acute or very recent infection as these are produced early by the immune system. The presence of IgG antibodies, on the other hand, may indicate either a past infection or an ongoing chronic infection. Measurement of IgG antibodies is often performed twice, a few weeks apart. A significant increase in levels implies an active infection. However, this interpretation can be complex due to possible cross-reactivity with other bacteria.

The Importance of Early Mycoplasma Detection

Early detection of Mycoplasma infection is of paramount importance due to several reasons. Firstly, early diagnosis allows for prompt treatment, controlling the spread of the infection and limiting the potential damage to the host. Secondly, in some cases, untreated Mycoplasma infections can lead to more severe conditions. For instance, Mycoplasma pneumoniae can cause serious respiratory illnesses in susceptible individuals and can lead to complications such as bronchitis and even pneumonia. Early detection is also crucial in research and development labs. Contamination by Mycoplasma in these settings can compromise the accuracy and reliability of experimental results. Another significant advantage of early detection lies in the prevention of transmission. Given the methods of transmission (respiratory droplets and sexual contact), timely diagnosis can significantly reduce the spread of these bacteria, particularly in residential and healthcare settings. In solution terms, the formula for diagnosis can be simply put as: \[ Diagnosis = Detection_{early} + Treatment_{timely} \] This equation emphasizes the importance of early detection, along with timely treatment, in managing this unique and intriguing group of bacteria - the Mycoplasma.

Prevention and Management of Mycoplasma Infections

When it comes to managing and preventing Mycoplasma infections, understanding the nature, causes, risk factors, and modes of transmission is essential. Here, you'll be introduced to a range of proactive preventive measures primarily for two significant Mycoplasma species - Mycoplasma genitalium and Mycoplasma pneumoniae.

Proactive Measures Against Mycoplasma Genitalium

Mycoplasma genitalium is a type of bacteria commonly associated with sexually transmitted infections (STIs).

It is deemed vital to focus on prevention, considering the concerns centred around the increasing antibiotic resistance of this bacterium. Effective preventive measures can play a crucial role in controlling its further spread.
  • Safe Sex Practices: Since Mycoplasma genitalium is primarily sexually transmitted, practising safe sex is most important. This encompasses consistent and correct use of latex or polyurethane condoms and dental dams during all sexual activities. Infection control begins with understanding that barrier methods are critical in preventing direct contact with body fluids that may be carrying the bacteria.
  • Regular Screening: Regular sexual health check-ups and prompt testing for STIs play a significant role in early detection, treatment and limitation of transmission. It is especially beneficial for sexually active individuals with multiple partners or those with a diagnosed STI.
  • Partnership Communication: Open and honest communication with sexual partners about STI testing, risk factors, and safer sex practices can help manage and mitigate the risk of Mycoplasma genitalium infection.
In parallel, understanding the management of Mycoplasma genitalium infection is vital post-diagnosis. Assured adherence to a complete course of the prescribed antibiotic regime is mandatory to minimise the risk of developing antibiotic resistance. It may also be beneficial to abstain from any sexual activity until the infection is completely cleared to prevent spreading it to others. A mathematical look into the equation of prevention and management could be noted as: \[ Prevention \, of \, Infection = Safe \, Sex Practices + Regular \, Screening + Partnership \, Communication + Proper \, Treatment \]

How to Handle a Mycoplasma Pneumoniae Infection Effectively

Mycoplasma pneumoniae is a bacterial species that predominantly leads to atypical pneumonia infections. The bacteria spreads through respiratory droplets, making close living conditions like schools and care homes potential hotspots for outbreaks. Here are some steps for effective handling of a Mycoplasma pneumoniae infection:
  • Basic Hygiene Practices: Regular hand washing with soap or use of sanitiser, coupled with minimising face touching, can lower the risk of acquiring or spreading the infection.
  • Respiratory Hygiene: Using a tissue or the elbow to cover the mouth and nose during a cough or a sneeze can prevent the wide spread of respiratory droplets.
  • Avoidance of Close Contact: Minimising close contact with infected individuals and staying at home when sick are vital in controlling the transmission.
  • Healthy Living: Keeping a healthy immune system by maintaining a balanced diet, regular exercise, and sufficient sleep can help the body fend off infections including Mycoplasma pneumoniae.
When it comes to managing an established Mycoplasma pneumoniae infection, prompt medical attention and adherence to treatment regimens is crucial. Doctors generally prescribe a course of antibiotics, often macrolides or tetracyclines, aimed at slowing the growth of or killing the bacteria. The effective handling of Mycoplasma pneumoniae infections can be summarised by this equation: \[ Handling \, Infection = Basic \, Hygiene + Respiratory \, Hygiene + Avoidance \, of \, Close \, Contact + Healthy \, Living + Proper \, Treatment \] However, it's important to remember that prevention strategies are based on reducing exposure to the bacterium and that managing an infection is largely dependent on medical advice and intervention. Carry on reading to go further into this interesting and impactful exploration of Mycoplasma.

Recent Developments in Mycoplasma Research

Being one of the smallest and simplest life forms, Mycoplasma has drawn increasing interest from the scientific community. Today, we'll delve deeper into the latest advancements in Mycoplasma research, specifically focusing on two species with significant implications for human health: Mycoplasma genitalium and Mycoplasma pneumoniae.

Advancements in Mycoplasma Genitalium Research

Mycoplasma genitalium is a bacterium associated, primarily, with sexually transmitted infections (STIs). Despite the knowledge of its existence since the 1980s, our understanding of this bacterium has been limited due to its demanding culture requirements. In recent years, however, breakthroughs in research methodologies have paved the way for more effective investigation and management strategies. First off, significant strides have been made in developing more robust and reliable testing methods for the detection of Mycoplasma genitalium. Techniques such as multi-colour fluorescence in situ hybridisation (FISH) and advanced PCR methodologies now permit speedier results while maintaining high levels of specificity and sensitivity. These have been particularly useful in monitoring antibiotic resistance, facilitating timely patient management. Research into the bacterial growth patterns of Mycoplasma genitalium has shone light on innovative avenues for therapeutic intervention. For example, the bacterium's unique dependence on cholesterol for growth has led to proposals for cholesterol depletion as a potential new treatment avenue. Known as "starvation therapy", this approach could offer an alternative means to combat antibiotic resistance. Meanwhile, understanding the mechanisms by which Mycoplasma genitalium invades and inflames host tissues has furnished insights into the bacterium's pathogenicity. This has supported the development of targeted therapies, focusing on hindering specific bacterial processes, such as protein adhesion, to limit tissue infiltration. Resistant strains of Mycoplasma genitalium pose a grave threat, prompting significant research into antibiotic stewardship. This entails optimising drug regimens to limit the emergence and spread of resistant strains. This research aids clinicians in deciding the preferred line of treatment, based on evidence of the bacterium's evolving susceptibility patterns.

New Understandings in Mycoplasma Pneumoniae Studies

Mycoplasma pneumoniae is a bacterium notorious for causing atypical pneumonia. Its significance in upper and lower respiratory tract infections has led to a wealth of research in this area. A progressive understanding of the bacterial pathogenesis of Mycoplasma pneumoniae is being accumulated through advanced studies in genetic and molecular biology. Learning how the bacterium adheres to lung tissues, initiates infection, and circumvents the human immune system, has furnished ways to intervene during these stages, thereby reducing the severity of infection. Significant advancements have also been made in diagnostic methodologies. The incorporation of real-time PCR and other molecular techniques in diagnostic testing allows for the distinct identification of this bacterium, creating further opportunities for early intervention and prevention of bacterial spread. There has been an explosion of new knowledge regarding antibiotic resistance in Mycoplasma pneumoniae, especially with regards to the macrolide class of antibiotics. Researchers are now able to better understand the specific genetic changes in Mycoplasma pneumoniae that account for this resistance. These findings prompt critical modifications in treatment guidelines and stimulate the pursuit for alternate therapeutic agents. Lastly, research is expanding in understanding the long-term complications of Mycoplasma pneumoniae infections. This includes potential links between Mycoplasma pneumoniae and diseases such as asthma, rheumatoid arthritis, and neurological disorders. Recognising these connections could lead to better patient management strategies and improved healthcare outcomes. In sum, the ongoing evolution of research into Mycoplasma genitalium and Mycoplasma pneumoniae is augmenting our understanding of these species, informing and evolving diagnostic strategies, therapeutic interventions, public health guidelines, and shaping future prevention efforts in microbiology.

Mycoplasma - Key takeaways

  • Mycoplasma genitalium and Mycoplasma pneumoniae are two main species of Mycoplasma, causing infections in the urogenital tract and respiratory system, respectively.
  • Mycoplasma infections often manifest with species-specific symptoms, such as urethritis in men and cervicitis in women for Mycoplasma genitalium, and symptoms akin to a common cold or flu for Mycoplasma pneumoniae.
  • Typical antibiotics do not effectively treat Mycoplasma infections due to their lack of a cell wall. Instead, antibiotics that target protein synthesis, like macrolides and tetracyclines, are used.
  • Mycoplasma testing is essential for the accurate diagnosis of infections, with methods including culture tests, serological tests, and molecular tests such as PCR.
  • Early detection of Mycoplasma infection is crucial for prompt treatment, prevention of more severe conditions, and reducing the chance of spreading these bacteria.

Frequently Asked Questions about Mycoplasma

Mycoplasma is a genus of bacteria that lack a cell wall around their cell membrane. This unique feature makes them unaffected by many common antibiotics such as penicillin. They are the smallest bacterial cells yet discovered, and are typically parasitic or saprotrophic.

Mycoplasma genitalium is a sexually transmitted bacteria first identified in 1980. It infects the urethra in men and the reproductive tract in women, often causing inflammation and potentially serious reproductive complications. The infection often presents with no symptoms, making it difficult to diagnose.

Mycoplasma is typically treated with antibiotics such as doxycycline, erythromycin, or azithromycin. In some cases, combination therapy may be necessary. Consultation with a healthcare professional is vital for appropriate treatment.

Mycoplasma hominis infection is commonly treated with antibiotics, such as doxycycline, clindamycin, or fluoroquinolones. A healthcare professional will typically prescribe a course of treatment. However, some strains may be resistant, requiring alternative antibiotics. Always follow your doctor's advice for treatment.

Mycoplasma are not technically classified as Gram-negative because they lack the cell wall, which is needed for the Gram staining process. However, they do have similar traits to Gram-negative bacteria such as sensitivity to certain antibiotics.

Final Mycoplasma Quiz

Mycoplasma Quiz - Teste dein Wissen

Question

What is Mycoplasma and what makes it unique?

Show answer

Answer

Mycoplasma is a genus of bacteria that lacks a cell wall, making them resistant to many common antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic and exist in almost every habitat where there is life.

Show question

Question

What are the two most studied species of Mycoplasma and why are they significant in research?

Show answer

Answer

The two most studied species of Mycoplasma are Mycoplasma genitalium, which is used as a model organism due to its small genome, and Mycoplasma pneumoniae, which offers valuable insights into bacterial pathogenesis and host-adaptation mechanisms.

Show question

Question

What role does Mycoplasma play in the field of microbiology?

Show answer

Answer

Mycoplasma plays a crucial role in microbiology, providing important insights about bacterial life and antibiotic resistance due to its unique properties, such as lacking a cell wall and its ability to withstand different habitats.

Show question

Question

What symptoms are typically associated with Mycoplasma pneumoniae and Mycoplasma genitalium infections?

Show answer

Answer

Mycoplasma pneumoniae typically causes respiratory infections with symptoms akin to cold or flu, like dry cough and sore throat, and can include chest pain and laboured breathing as the infection progresses. Mycoplasma genitalium affects the urogenital tract, with men experiencing urethritis symptoms and women displaying symptoms of cervicitis.

Show question

Question

How is a Mycoplasma infection caused and treated?

Show answer

Answer

A Mycoplasma infection happens when you're exposed to the bacteria, often via respiratory droplets, or sexual contact. Because Mycoplasmas lack a cell wall, typical antibiotics are ineffective. Instead, antibiotics targeting protein synthesis like macrolides or tetracyclines, and sometimes quinolones, are used. The treatment duration can range from 5-14 days.

Show question

Question

What is the impact of Mycoplasma hominis infections?

Show answer

Answer

Mycoplasma hominis generally lives in the urinary and genital tracts and can be problematic under certain conditions. In women, it can cause pelvic inflammatory disease and, in pregnant women, can lead to amnionitis and postpartum fever. In men, it can cause non-gonococcal urethritis. Mycoplasma hominis is also resistant to many antibiotics, including tetracyclines.

Show question

Question

What kind of samples are collected for Mycoplasma testing?

Show answer

Answer

Depending on the suspected site of infection, sputum samples, throat swabs, urine samples, or swabs from the affected genital area can be collected.

Show question

Question

How is a Mycoplasma infection diagnosed in a laboratory?

Show answer

Answer

A culture test is usually done first to identify the growth of Mycoplasma bacteria. Following this, serological tests detect the body's immune response and molecular tests identify the presence of Mycoplasma DNA or RNA.

Show question

Question

Why is early detection of Mycoplasma infection important?

Show answer

Answer

Early detection allows for prompt treatment preventing the spread of the infection, possible development of severe conditions, compromising of experimental results in labs, and reduction of the transmission in residential and healthcare settings.

Show question

Question

What are some proactive measures against Mycoplasma genitalium?

Show answer

Answer

To prevent Mycoplasma genitalium, you should practice safe sex, get regular sexual health check-ups, have open communication with sexual partners, and adhere to prescribed antibiotic regimes.

Show question

Question

How can you handle a Mycoplasma pneumoniae infection effectively?

Show answer

Answer

Handling a Mycoplasma pneumoniae infection involves practicing basic and respiratory hygiene, avoiding close contact with infected individuals, leading a healthy lifestyle, and adhering to treatment regimens that often include antibiotics.

Show question

Question

What is the nature of Mycoplasma genitalium and how is it transmitted?

Show answer

Answer

Mycoplasma genitalium is a bacteria associated with sexually transmitted infections (STIs) and is primarily spread through sexual contact.

Show question

Question

What recent advancement has been made in detecting Mycoplasma genitalium infections?

Show answer

Answer

The development of more robust and reliable testing methods such as multi-colour fluorescence in situ hybridisation (FISH) and advanced PCR methodologies for quicker results while maintaining specificity and sensitivity.

Show question

Question

What are the implications of understanding the bacterial growth patterns of Mycoplasma genitalium?

Show answer

Answer

It has led to the proposal of cholesterol depletion, or "starvation therapy", as a potential new treatment avenue to combat antibiotic resistance.

Show question

Question

What new insight has been obtained about Mycoplasma pneumoniae's antibiotic resistance?

Show answer

Answer

Researchers understand the specific genetic changes in Mycoplasma pneumoniae that account for resistance, especially regarding the macrolide class of antibiotics, prompting modifications in treatment guidelines and the search for alternate therapeutic agents.

Show question

Test your knowledge with multiple choice flashcards

What is Mycoplasma and what makes it unique?

What are the two most studied species of Mycoplasma and why are they significant in research?

What role does Mycoplasma play in the field of microbiology?

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Flashcards in Mycoplasma15

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What is Mycoplasma and what makes it unique?

Mycoplasma is a genus of bacteria that lacks a cell wall, making them resistant to many common antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic and exist in almost every habitat where there is life.

What are the two most studied species of Mycoplasma and why are they significant in research?

The two most studied species of Mycoplasma are Mycoplasma genitalium, which is used as a model organism due to its small genome, and Mycoplasma pneumoniae, which offers valuable insights into bacterial pathogenesis and host-adaptation mechanisms.

What role does Mycoplasma play in the field of microbiology?

Mycoplasma plays a crucial role in microbiology, providing important insights about bacterial life and antibiotic resistance due to its unique properties, such as lacking a cell wall and its ability to withstand different habitats.

What symptoms are typically associated with Mycoplasma pneumoniae and Mycoplasma genitalium infections?

Mycoplasma pneumoniae typically causes respiratory infections with symptoms akin to cold or flu, like dry cough and sore throat, and can include chest pain and laboured breathing as the infection progresses. Mycoplasma genitalium affects the urogenital tract, with men experiencing urethritis symptoms and women displaying symptoms of cervicitis.

How is a Mycoplasma infection caused and treated?

A Mycoplasma infection happens when you're exposed to the bacteria, often via respiratory droplets, or sexual contact. Because Mycoplasmas lack a cell wall, typical antibiotics are ineffective. Instead, antibiotics targeting protein synthesis like macrolides or tetracyclines, and sometimes quinolones, are used. The treatment duration can range from 5-14 days.

What is the impact of Mycoplasma hominis infections?

Mycoplasma hominis generally lives in the urinary and genital tracts and can be problematic under certain conditions. In women, it can cause pelvic inflammatory disease and, in pregnant women, can lead to amnionitis and postpartum fever. In men, it can cause non-gonococcal urethritis. Mycoplasma hominis is also resistant to many antibiotics, including tetracyclines.

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